Hematoxylin Solution is a nuclear stain that is used as a counterstain for immunohistochemistry (IHC). Together with eosin, it forms the Hematoxylin and Eosin stain (H&E stain), one of the most commonly used stains in histology.

A common laboratory method uses two dyes called hematoxylin and eosin which make it easier to see different parts of the cell under a microscope. Hematoxylin shows the ribosomes, chromatin (genetic material) within the nucleus, and other structures as a deep blue-purple color.

Hematoxylin is a dye commonly used in histology to stain cell nuclei, making it easier to visualize tissue structures under a microscope. Here’s a basic overview of the hematoxylin solution:

Components of Hematoxylin Solution:

• Hematoxylin: The primary dye derived from the logwood tree.
• Ethanol: Often used as a solvent.
• Water: Typically added to dilute the solution.
• Stabilizers: Sometimes included to enhance shelf life.

Preparation:

1. Dissolve Hematoxylin: Mix the hematoxylin powder with ethanol or a suitable solvent.
2. Dilute: Add distilled water to achieve the desired concentration (commonly 0.5% to 1%).
3. Add Mordant (optional): To enhance staining, a mordant like aluminum sulfate may be included.

Usage of Hematoxylin Solution:

• Staining Procedure:
  1. Deparaffinize and hydrate the tissue sections.
  2. Immerse sections in hematoxylin for a specified time (usually a few minutes).
  3. Rinse with water.
  4. Differentiate with an acid-alcohol solution if necessary.
  5. Rinse again and counterstain with eosin if desired.

Storage:

• Store in a dark, cool place to prevent degradation of the dye.

Safety:

• Handle with care, as concentrated solutions can be hazardous. Use gloves and goggles.